Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Jan 1 2015
Last update date: May 15 2017
Access: Public
Diseases: Breast Neoplasms, Neoplasms, Second Primary
Dataset link The N-glycosylated isoform of β-integrin as a potential biomarker and target in HER-2+ BC refractory to HER-2 targeted therapies.

Experimental Protocol


RNA was isolated from 10 breast cancer cell lines in triplicate. Pairwise gene expression differences were compared between each of the SKBR-3 cell lines (SKBR-3/ b1, SK-EV-C4, and SK-B1-C1). Features selected had at least a 2X difference in at least one comparison, with a one-way ANOVA corrected p-value of >0.05. In addition, expression profiles from SKBR-3/ EV and SK-B1-C1 cell were compared and a differential gene list was generated to include genes that differed by at least 1.5X, with a t-test p-value of >0.02 (unpaired, Bonferroni corrected). A total of 1940 entities met these criteria and the expression of these genes was investigated in all breast cancer cell lines.

Repositories


GEO

GSE40152

ArrayExpress

E-GEOD-40152

BioProject

PRJNA173016

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