|Application:||RNA-seq analysis, sRNA-seq analysis|
|Number of samples:||2|
|Release date:||Jul 19 2014|
|Last update date:||Jul 20 2014|
|Dataset link||Deciphering microRNAs and other small RNAs during the transition of dormant embryo into germinated embryo in larch (Larix leptolepis)|
One embryogenic cell line of Japanese larch (Larix leptolepis), designated as D878, with a high embryo maturation capacity was used in this study. Embryogenic callus were induced from immature embryos of larch on induction medium, followed by sub-culture, and culture on ABA-containing mature medium in a dark environment at 25 2 C. After cultured 45 days in mature medium, embryogenic calli developed into mature somatic embryos. In our study, the samples were harvested at day 57, one sample was collected after mature embryos continued to stay for 12 days on ABA-containing medium, and the other one was harvested after cultured for 12 days on ABA-removing medium. All samples were snap-frozen in liquid nitrogen, and stored in liquid nitrogen until RNA extraction.