em2em specifications

Information


Unique identifier OMICS_26600
Name em2em
Software type Application/Script
Interface Command line interface, Graphical user interface
Restrictions to use None
Input format BROOKHAVEN STEM,CCP4,DATA ONLY,DIG. MICROGRAPH,EM,FEI MRC,FEI MRCS,FEI RAW IMAGE,FORMATTED,IMAGIC,JPEG,KONTRON,MDPP,MEDIPIX,MRC,OFFSET,PIF,PGM,POSTSCRIPT, RAWIV/VOLUMETRIC,RGB_TIFF,SHF,SITUS,SPIDER,SUPRIM,TIA (EMISPEC),TIFF,TVIPS,X-PLOR
Operating system Unix/Linux, Mac OS, Windows
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


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Maintainer


  • person_outline em2em Team <>

Additional information


em2em is combined with FSC in a single download archive.

em2em in publications

 (4)
PMCID: 5883021
PMID: 29615809
DOI: 10.1038/s41598-018-23816-2

[…] 20 kv under low-dose conditions. images of filaments were acquired by a 4 k × 4 k ccd camera (eagle, fei). long, relatively straight filaments were unbent using imagej and converted to spider format (em2em; image science and imperial college, london, uk). selected filaments were cut into segments in spider, and iterative helical real space reconstruction (ihrsr) was carried out using spider. an f- […]

PMCID: 5805719
PMID: 29422607
DOI: 10.1038/s41598-018-21053-1

[…] long and relatively straight filaments were selected for 3d reconstruction and straightened with imagej (nih), as previously described. straightened filaments were converted to spider format (em2em; image science and imperial college, london, uk) and cut into segments in spider (v11.2, wadsworth center, albany, ny). iterative helical real-space reconstruction (ihrsr) was carried […]

PMCID: 4380226
PMID: 25839057
DOI: 10.1126/sciadv.1400205

[…] on the basis of the increase in their diameter due to bound c0c3 (). long, relatively straight filaments were unbent using imagej (national institutes of health) and converted to spider format (em2em; image science and imperial college, london). a density projection along the filament axis was obtained using the spider (v11.2) software package () to determine the diameter calculated […]

PMCID: 2531245
PMID: 18472277
DOI: 10.1016/j.jsb.2008.03.011

[…] format and were then transferred to a linux unix operating system pc running the imagic suite of programs () to analyse them. initially, they were converted to floating point imagic format using em2em. the resulting images were then binned by a factor of 4 in both the x and y directions, resulting in a final step size of 6.35 å/pixel and converted to mrc format for pre-processing using […]


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