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Ephus specifications


Unique identifier OMICS_19743
Name Ephus
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Windows
Programming languages MATLAB
Computer skills Medium
Version 2.2.0
Stability Stable
Maintained Yes


No version available


  • person_outline Gordon Shepherd

Additional information


Publication for Ephus

Ephus citations


Directional Reaching for Water as a Cortex Dependent Behavioral Framework for Mice

Cell Rep
PMCID: 5863030
PMID: 29514103
DOI: 10.1016/j.celrep.2018.02.042
call_split See protocol

[…] w was illuminated with a collimated red LED light (630 nm) and imaged at 10 fps with 256-by-332 pixel resolution using 12-bit camera (QImaging). Stimulation and image acquisition were controlled with Ephus (http://scanimage.vidriotechnologies.com/display/ephus/Ephus). Twenty post-stimulus frames were averaged and subtracted to baseline average image (1.5 s before stimulus). Ten to 20 repetitions w […]


Circuit specific and neuronal subcellular wide E I balance in cortical pyramidal cells

Sci Rep
PMCID: 5838226
PMID: 29507308
DOI: 10.1038/s41598-018-22314-9

[…] .35 with CsOH (289 mOsm). Whole-cell recordings were made with an Axopatch 700B amplifier (Axon Instruments) and a 1322 A board. Data from CRACM experiments were collected using Matlab-based software Ephus. Cells below pia surface ranged 50 to 110 µm were collected. For CRACM, EPSCs were recorded in voltage clamp with holding potential at −45 to −48 mV (L2/3 cells) or −45 to −55 mV (L5B cells) suc […]


Methodological Approach for Optogenetic Manipulation of Neonatal Neuronal Networks

PMCID: 5554786
PMID: 28848399
DOI: 10.3389/fncel.2017.00239

[…] s (tip resistance of 4–7 MΩ) filled with potassium-based pipette solution (in mM: 130 K-Gluconate, 10 HEPES, 0.5 EGTA, 4 Mg ATP, 0.3 Na GTP, 8 NaCl; 285 mOsm, pH 7.4). Recordings were controlled with Ephus software in Matlab environment (MathWorks, MA, USA). Capacitance artifacts were minimized using the built-in circuitry of the patch-clamp amplifier (Axopatch 200B, Molecular devices, Sunnyvale, […]


Reorganization of corticospinal output during motor learning

Nat Neurosci
PMCID: 5656286
PMID: 28671694
DOI: 10.1038/nn.4596
call_split See protocol

[…] red with Scanimage 4.1 (Vidrio Technologies) at a rate of ~28 Hz, covering ~340 μm × 340 μm with 512×512 square pixels. Frame triggers, lever voltage, and the start times of trials were recorded with Ephus (Vidrio Technologies), allowing for alignment between behavior and imaging. Drifts in the imaging field during imaging were manually monitored and corrected. Images were motion corrected offline […]


Intracortical Circuits in Thalamorecipient Layers of Auditory Cortex Refine after Visual Deprivation

PMCID: 5383732
PMID: 28396883
DOI: 10.1523/ENEURO.0092-17.2017

[…] –9 MΩ. Cells targeted for recording are located in an area of A1 overlying the rostral flexure of the hippocampus. Data acquisition is performed by National Instruments AD boards and custom software (Ephus; ), which is written in Matlab (Mathworks) and adapted to our setup. Voltages are corrected for an estimated junction potential of 10 mV. Electrodes are filled with (in mm) 115 cesium methanesul […]


Stage specific functions of Semaphorin7A during adult hippocampal neurogenesis rely on distinct receptors

Nat Commun
PMCID: 5353663
PMID: 28281529
DOI: 10.1038/ncomms14666

[…] cytin (Sigma) and 10 μM Alexa Fluor 594 dye (Invitrogen).Whole-cell patch-clamp recordings were acquired at 10 kHz using a Multiclamp 700B with an online 2 kHz low pass filter (Molecular Devices) and Ephus software (www.ephus.org). Data analysis was performed in Matlab, R (http://cran.r-project.org), Igor Pro (Wavemetrics) and Excel (Microsoft). Recordings were corrected for a liquid junction pote […]


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Ephus institution(s)
Department of Physiology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA; Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA

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