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Expressed sequence tag annotation software tools | Transcription data analysis

Expressed sequence tags (EST) represent short, unedited, randomly selected single-pass sequence reads derived from cDNA libraries, providing a low-cost alternative (also called ‘poor’ man's genome) to whole genome sequencing (Adams et al., 1992; Nagaraj et al., 2007) and specifically relevant to the transcriptome of an organism at various stages of development or under different experimental conditions. The analysis of EST data can enable gene discovery, complement genome annotation, aid gene structure identification, establish the viability of alternative transcripts, guide single nucleotide polymorphism (SNP) characterization and facilitate proteomic exploration (Nagaraj et al., 2007). Source text: Nagaraj et al., 2007.

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