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ExpressYourself

A web-based program for processing microarray data. In completely automated fashion, ExpressYourself will correct the background array signal, normalize the Cy5 and Cy3 signals, score levels of differential hybridization, combine the results of replicate experiments, filter problematic regions of the array and assess the quality of individual and replicate experiments. ExpressYourself is designed with a highly modular architecture so various types of microarray analysis algorithms can readily be incorporated as they are developed; for example, the system currently implements several normalization methods, including those that simultaneously consider signal intensity and slide location. The processed data are presented using a web-based graphical interface to facilitate comparison with the original images of the array slides. In particular, Express Yourself is able to regenerate images of the original microarray after applying various steps of processing, which greatly facilities identification of position-specific artifacts.

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ExpressYourself classification

ExpressYourself specifications

Interface:
Web user interface
Computer skills:
Basic
Maintained:
Yes
Restrictions to use:
None
Stability:
Stable

ExpressYourself distribution

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Publications

Institution(s)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven CT, USA; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven CT, USA; Department of Statistics, Yale University, New Haven CT, USA

Link to literature

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