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Protocols

Extended Depth of Field specifications

Information


Unique identifier OMICS_25628
Name Extended Depth of Field
Software type Package/Module
Interface Graphical user interface
Restrictions to use Academic or non-commercial use
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
Computer skills Medium
Version 10.11.2015
Stability Stable
Requirements
ImageJ
Maintained Yes

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Versioning


No version available

Maintainer


  • person_outline Daniel Sage

Additional information


http://imagej.net/Extended_Depth_of_Field

Extended Depth of Field citation

call_split

A Division in PIN Mediated Auxin Patterning during Organ Initiation in Grasses

2014
PLoS Comput Biol
PMCID: 3907294
PMID: 24499933
DOI: 10.1371/journal.pcbi.1003447
call_split See protocol

[…] ith ImageJ (http://rsbweb.nih.gov/ij/). Citrine was excited at 514 nm and mRFP at 561 nm. The pinhole was set to one Airy unit. For each z-stack, transmitted light was detected and flattened using an extended-depth-of-field plugin (http://bigwww.epfl.ch/demo/edf/). Fluorescent channels were processed with a median filter to reduce noise and were recombined with the processed transmitted light imag […]


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