Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Dec 30 2013
Last update date: Nov 9 2017
Access: Public
Diseases: Carcinoma, Non-Small-Cell Lung, Neoplasms
Dataset link Recombinant human lactoferrin activates human dendritic cells via Toll-like receptors-2 and -4

Experimental Protocol


Human peripheral blood mononuclear cells (PBMCs) were obtained from buffy coats of healthy donors. CD14+ cells were isolated from fresh PBMCs by positive immunoselection with magnetic beads. Immature dendritic cells (i-DC) from four donors were generated using granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. On day 6, cells were harvested in order to have iDC or mDC (conventionally matured DC) by addition of Tumor Necrosis Factor (TNF) -alfa and IL-1beta or TLF-DC (Talactoferrin matured DC) adding Food and Drug Administration-approved, GMP level recombinant TLF generated in eukaryotic cells. After 24 hour culture, cells were harvested and analyzed with Illumina arrays.

Repositories


GEO

GSE26438

ArrayExpress

E-GEOD-26438

BioProject

PRJNA136819

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Contact


Raffaele Calogero
Raffaele A Calogero