Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Jun 14 2011
Last update date: Mar 23 2012
Access: Public
Diseases: Neoplasms
Dataset link Glycan analysis of promary human lymphatic endothlial cells (LEX) in their resting and activated states, in normal and tumor tissues

Experimental Protocol


We would anticipate that changes in glycosylation of LEC cell surface proteins following activation might affect important functions associated withy LEC including eg. interactions with leukocytes and/or sequestration of GAG-binding chemokines. We already have shown that ligand binding to the lymphatic endothelial hyaluronan receptor LYVE-1 is reversibly masked by terminal sialation in LEC and that functional regulation of LYVE-1 is likely to be important in inflammation. An advantage of our proposal is that we can routinely isolate primary LEC in relatively large numbers, and have the necessary ethical approval to do so from human tissue. Glycan analysis of primary human lymphatic endothelial cells (LEC) in their resting and activated states, in normal and tumour tissues and a comparison of the LEC glycan structures with those from blood vessel endothelial cells

Repositories


GEO

GSE29933

ArrayExpress

E-GEOD-29933

BioProject

PRJNA140879

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Contact


Steven Head