Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Jan 22 2009
Last update date: Mar 19 2012
Access: Public
Taxon: Sus scrofa, Homo sapiens
Dataset link Heart-muscle expression ratios in pig obtained by 454-sequencing, iTRAQ-based proteomics and cDNA microarrays

Experimental Protocol


Tissue samples of heart (HEA) and Longissimus dorsi (LDO) were prepared by pooling from five healthy Hampshire gilts at age four to six months and division into six sub-samples, three for each tissue named HEA1, HEA2, HEA3, LDO1, LDO2 and LDO3. The exact same six tissue samples were used for subsequent expression profiling with cDNA microarray, 454-sequencing and iTRAQ-based proteomics. A reference sample for the cDNA microarray experiment and iTRAQ-based proteomics was constructed by combining the six samples. In the microarray experiment, three cDNA microarray slides were used per sample.

Repositories


GEO

GSE10122

ArrayExpress

E-GEOD-10122

BioProject

PRJNA108665

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Contact


Jakob Hedegaard

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