Dataset features

Specifications


Dataset type: Non-coding RNA profiling by array
Number of samples: 21
Release date: Jan 7 2019
Last update date: Feb 4 2019
Access: Public
Dataset link Long non-coding RNAs expressed in fast gycolytic and slow oxidative myofibers

Experimental Protocol


EDL and soleus mouse muscles were incubated with type I collagenase (Sigma-Aldrich) to dissociate intact myofibers that were separately collected under stereomicroscope. Isolated myofibers were divided in two parts: one was immersed in Laemmli buffer for fiber typing; the other was placed in TRIzol Reagent (Life Technologies) for RNA purification. Myofibers were classified according to their myosin heavy chain (MyHC) protein isoform in pure MyHC-1, and -2B. We analyzed the transcription profiles of 10 single isolated myofibers (biological replicas) for type 2B myofibers and 11 single isolated myofibers (biological replicates) for type 1 myofibers (total: 21 experiments). One-color hybridizations were performed on SurePrint G3 Mouse GE 8x60K Microarrays (Agilent Technologies). Microarray platform was re-annotated according transcripts in Ensembl 74. Only long non coding RNAs were considered for expression analyses.

Repositories


GEO

GSE112716

BioProject

PRJNA448779

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Contact


Gerolamo Lanfranchi