|Dataset type:||Non-coding RNA profiling by array|
|Number of samples:||21|
|Release date:||Jan 7 2019|
|Last update date:||Feb 4 2019|
|Dataset link||Long non-coding RNAs expressed in fast gycolytic and slow oxidative myofibers|
EDL and soleus mouse muscles were incubated with type I collagenase (Sigma-Aldrich) to dissociate intact myofibers that were separately collected under stereomicroscope. Isolated myofibers were divided in two parts: one was immersed in Laemmli buffer for fiber typing; the other was placed in TRIzol Reagent (Life Technologies) for RNA purification. Myofibers were classified according to their myosin heavy chain (MyHC) protein isoform in pure MyHC-1, and -2B. We analyzed the transcription profiles of 10 single isolated myofibers (biological replicas) for type 2B myofibers and 11 single isolated myofibers (biological replicates) for type 1 myofibers (total: 21 experiments). One-color hybridizations were performed on SurePrint G3 Mouse GE 8x60K Microarrays (Agilent Technologies). Microarray platform was re-annotated according transcripts in Ensembl 74. Only long non coding RNAs were considered for expression analyses.