|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||May 23 2012|
|Last update date:||May 24 2012|
|Dataset link||HoxA13 gain-of-function in chicken embryo PSM progenitors.|
Primitive streak aera corresponding to PSM precursors were dissected in Stage 9 somites chicken embryo overexpressing HoxA13 or a control H2B-Venus. The experiment was designed to have biological duplicate in each conditions. The gain-of-function was obtained by electroporating the embryo at Stage 5HH with a vector containing HoxA13 under the CAGGS promoter with an H2B venus reporter. In order to have enough material for the microarray, 7 embryos were pooled in each sample before the hybridization.