Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Feb 15 2012
Last update date: Mar 23 2012
Access: Public
Dataset link Comparative analysis of stylar canal cells transcriptome in clementine mandarin (Citrus clementina)

Experimental Protocol


Styles with stigmas, collected 24 hours after self pollination, were immediately snap-frozen in OCT embedding medium (Sakura Finetek, Zoeterwoude, Netherlands) in Peel-A-Way plastic embedding molds (Polysciences, Polysciences, Warrington, PA, USA). Transversal sections 10 µm thick at the upper part of the style were cut with a Leica CM1900 cryostat (Leica Microsystems, Germany) at -20°C. A Leica AS Laser Microdissection system (Leica Microsystems) was used for the isolation of stylar canals from transversal sections. Canals from the stigma were discarded to avoid contamination with pollen or pollen tubes. Three biological replicates were prepared for each genotype. Each biological replicate consisted of bulks of about 200 microdissected areas (composed of an average of 50 cells) coming from two different molds

Repositories


GEO

GSE33014

ArrayExpress

E-GEOD-33014

BioProject

PRJNA148041

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Contact


Marco Caruso
Paz Merelo

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