Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 11
Release date: Jan 1 2008
Last update date: Mar 16 2012
Access: Public
Taxon: Danio rerio
Dataset link Identification of sexually dimorphic gene expression in the brain of adult zebrafish (Danio rerio)

Experimental Protocol


Sexually mature fish (6 males and 6 females) were kept in a 15L tank and allowed to breed naturally. After a 20-day period, fish were sacrificed humanly and the brain was extracted, fixed in RNAlater (Ambion) and stored at -20oC until required for microarray analysis. RNA was extracted using the TRI reagent method followed by a LiCl precipitation (Sigma) according to the manufacturer's instructions. Total RNA was amplified and labeled using the Amino Allyl MessageAmp aRNA amplification kit (Ambion). A reference design was used for the array hybridizations. Array analysis was performed according to established protocols (described in Santos et al., 2007) and the data was extracted and queried for differences in gene expression profiles between genders.

Repositories


GEO

GSE6853

ArrayExpress

E-GEOD-6853

BioProject

PRJNA99093

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Contact


Peter Kille
Eduarda Santos
Eduarda M Santos