|Application:||Gene expression microarray analysis|
|Number of samples:||11|
|Release date:||Jan 1 2008|
|Last update date:||Mar 16 2012|
|Dataset link||Identification of sexually dimorphic gene expression in the brain of adult zebrafish (Danio rerio)|
Sexually mature fish (6 males and 6 females) were kept in a 15L tank and allowed to breed naturally. After a 20-day period, fish were sacrificed humanly and the brain was extracted, fixed in RNAlater (Ambion) and stored at -20oC until required for microarray analysis. RNA was extracted using the TRI reagent method followed by a LiCl precipitation (Sigma) according to the manufacturer's instructions. Total RNA was amplified and labeled using the Amino Allyl MessageAmp aRNA amplification kit (Ambion). A reference design was used for the array hybridizations. Array analysis was performed according to established protocols (described in Santos et al., 2007) and the data was extracted and queried for differences in gene expression profiles between genders.
Eduarda M Santos