Computational protocol: Profiling of the Mammalian Mitotic Spindle Proteome Reveals an ER Protein, OSTD-1, as Being Necessary for Cell Division and ER Morphology

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Protocol publication

[…] Images were captured on a 200M inverted, Axioscope microscope (Carl Zeiss) equipped with a spinning disk confocal scan head (QLC100; VisiTech, Sunderland, United Kingdom). For the multi-nucleate embryo screen, the ORCA ER camera (Hamamatsu) was operated through OpenLab software (Improvision, Coventry, United Kingdom) and MetaMorph software (Molecular Devices, LLC, Sunnyvale, CA, USA). For the detailed characterization of OSTD-1, the ORCA ER camera (Hamamatsu) was operated through MetaMorph version 7.7.7.0 (Molecular Devices, LLC, Sunnyvale, CA, USA). Images were taken every 10 s for all movies. ImageJ software [] was used for rotating and cropping TIFF files for movies and figures. […]

Pipeline specifications

Software tools MetaMorph, ImageJ
Application Microscopic phenotype analysis
Organisms Caenorhabditis elegans, Homo sapiens
Diseases Abnormalities, Drug-Induced, Neoplasms