Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 8
Release date: Apr 10 2018
Last update date: Oct 18 2018
Access: Public
Diseases: Glioblastoma
Chemicals: Estrogens
Dataset link Differential effects of estrogen receptor beta isoforms on glioblastoma progression

Experimental Protocol


Total RNA was isolated from U87 control, U87-ERβ-KO, U87-ERβ1 and U87-ERβ5 cells. Illumina TruSeq RNA Sample Preparation was performed following manufacturer's protocol. Samples were run on an Illumina HiSeq 3000 in duplicates. The combined raw reads were aligned to UCSC hg19 and genes were annotated by Tophat. Genes were annotated and quantified by HTSeq-DESeq pipeline.

Repositories


GEO

GSE104296

ENA

SRP118937

BioProject

PRJNA412261

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Contact


Yidong Chen