Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 14
Release date: Sep 3 2010
Last update date: Aug 23 2018
Access: Public
Dataset link Expression data from mouse wild-type and Dnmt1-/-Dnmt3a-/-Dnmt3b-/- embryonic stem(ES) cells and nuclear transfer-trophoblastic stem(ntTS) cells, and wild-type trophoblastic stem(TS) cells, primitive endoderm cells, and extra-embryonic endoderm(XEN) cells

Experimental Protocol


To investigate the properties of the TKO ntTS cells from their expression profiles, we compared the microarray data from the TKO ntTS cells with data from wild-type (WT) ntTS cells and other cell types that represent the three blastocyst-derived lineages: the epiblast (EPI), trophectoderm (TE), and primitive endoderm (PE). WT and TKO ES cells were used as EPI, TS cells derived from wild-type blastocysts were used as TE, and extra-embryonic endoderm (XEN) cells derived from wild-type blastocysts were used as PE. We also treated WT ES cells with Gata4 to induce parietal endoderm cells as PE. The total RNA was extracted from these cell lines, and microarray data were obtained using mouse expression microarrays.

Repositories


GEO

GSE20177

BioProject

PRJNA125647

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Contact


Morito Sakaue