Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Sep 19 2006
Last update date: Mar 16 2012
Access: Public
Diseases: Fatty Liver
Dataset link Livers of mice fed an atherogenic diet for 6 or 24 weeks

Experimental Protocol


For oligo-microarrays (AceGene Mouse Oligo Chip 30K; DNA Chip Research, Yokohama, Japan). Each microarray hybridization sample and the reference amino allyl aRNA were labeled with mono-reactive NHS esters of Cy5 and Cy3, respectively. Microarray hybridization and washing were performed according to the manufacturer’s instructions, and then scanned using an Agilent G2505B microarray scanner (Agilent Technologies, Palo Alto, CA, USA), followed by image analysis using GenePix Pro 4.1 software (Axon Instruments, Union City, CA, USA). Microarray data were normalized (LOWESS method) using GeneSpring v7.2 software (Agilent Technologies), and exported into tab-deliminated files for further analysis.

Repositories


GEO

GSE5852

BioProject

PRJNA97259

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Contact


Naoto Matsuzawa-Nagata