Dataset features

Specifications


Dataset type: Other
Number of samples: 13
Release date: Jan 12 2015
Last update date: Oct 16 2017
Access: Public
Chemicals: Hydroxyurea
Dataset link Break-Seq of DNA double stranded breaks in Saccharomyces cerevisiae WT and mec1 cells after exposure to hydroxyurea

Experimental Protocol


We queried the yeast genome for DSBs after cells were treated with 200 mM hydroxyurea during S phase. Samples were collected from 1) cells synchronized in G1 phase by alpha factor; 2) cells released from G1 into medium containing 200 mM hydroxyurea for 1 h; 3) cells recovering in fresh medium without hydroxyurea for 1 h after the 1 h exposure to HU. These samples are referred to as G1, HU 1h, and R 1h, respectively. The strains from which the samples were collected are indicated following the time point, e.g. G1_MEC1 or R 1h_mec1. The experiment with mec1 was done twice (Experiments A and B) and that with MEC1 was done once (Experiment C). In addition, a control experiment of in vitro digestion with BamHI using the G1_mec1 sample (G1_BamHI) was performed.

Repositories


GEO

GSE58808

ArrayExpress

E-GEOD-58808

ENA

SRP043557

BioProject

PRJNA253569

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Contact


Wenyi Feng