Computational protocol: A Comprehensive, High-Resolution Map of a Gene’s Fitness Landscape

Similar protocols

Protocol publication

[…] The RNAfold utility of the Vienna RNA Package (version 2.1.2) was used to predict the minimum free energy of RNA sequences (). For each allele in supplementary figure S7 (Supplementary Material online), the Gibbs free energy was calculated as the average free energy of every 39 nt window centered on nucleotides from −5 to +10 of the gene start as described (). […]

Pipeline specifications

Software tools RNAfold, ViennaRNA
Application RNA structure analysis
Organisms Escherichia coli