Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Dec 1 2010
Last update date: Mar 22 2012
Access: Public
Chemicals: Nitrates
Dataset link Genome-wide effect of cholera toxin and nitrate treatment in etiolated rice plants

Experimental Protocol


Total RNA was isolated from 10 day old excised rice leaves treated with or without cholera toxin in the presence of nitrate.Total RNA was first converted into cDNA and then into cRNA labeled with Cy3 and Cy5 dyes. These were hybridized on the 44K 60-mer whole genome arrays on glass slides. The slides were washed and scanned on an Agilent scanner (G2565B) at 100 % laser power. Data extraction was carried out with Agilent Feature Exiraction software (version 9.1). The experiment was repeated with a biological-cum-technical replicate where total RNA samples were isolated from a fresh batch of rice plants and the cRNAs were labeled with opposite dyes (dye-swap).

Repositories


GEO

GSE20924

ArrayExpress

E-GEOD-20924

BioProject

PRJNA124359

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Contact


Nandula Raghuram
Ravi Pathak