Computational protocol: An Aqueous Extract of Marine Microalgae Exhibits Antimetastatic Activity through Preferential Killing of Suspended Cancer Cells and Anticolony Forming Activity

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Protocol publication

[…] Anti-human Ki67 immunohistochemistry staining was performed using our lab's standard IHC protocol as previously described [, ]. For assessing the cell proliferation rate in primary tumors, Ki67-stained proliferating cells were documented by analyzing 10 randomly chosen high-power fields (40x) per tumor section (n = 6). The ImageJ plug-in “ImmunoRatio” was used to directly calculate the percentage of Ki67-positive cells in each image. For assessing pulmonary metastases, lung tissue sections from each host mouse were histologically examined for all Ki67-positive human-origin cancer cell foci under 20x magnification. Lung metastatic foci bigger than 50 microns were counted under 20x magnification across whole section areas. Lung surface areas were calculated using the counts of the total high-power fields (20x) in each section. […]

Pipeline specifications

Software tools ImageJ, ImmunoRatio
Applications Bright-field microscopy, Microscopic phenotype analysis
Organisms Homo sapiens, Mus musculus
Diseases Neoplasms, Pancreatic Neoplasms, Prostatic Neoplasms, Osteosarcoma, X-Linked Combined Immunodeficiency Diseases