Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Mar 9 2015
Last update date: Jul 15 2015
Access: Public
Dataset link Expression analysis of Pseudomonas aeruginosa PAO1 fatty acid synthesase II (FASII) deletions complemented by excess or limited expression of FASII genes accD, fabG, and fabF1

Experimental Protocol


A twelve chip study using total RNA recovered from three separate cultures of Pseudomonas aeruginosa regualted complemented FASII gene deletions under excess IPTG (high level expression of complementing gene copy) and three separate cultures under limited IPTG (low level expression of complementing gene copy). Each chip measures the expression level of 5,553 genes from Pseudomonas aeruginosa PAO1-LAC (ATCC47085) with fourteen 24-mer probe pairs (PM/MM) per gene, with probes on the array five times. Two biological replicates were done for each sample for a total of 12 chips.

Repositories


GEO

GSE66045

BioProject

PRJNA275770

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Contact


Donald Moir