Computational protocol: Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.

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Protocol publication

[…] Predicted protein sequences were analyzed and theoretical isoelectric points (pIs) and mass values of mature peptides were calculated using the PeptideMass program (http://us.expasy.org/tools/peptidemass.html). Amino acid sequence alignment was performed using DNAMAN software. A phylogenetic tree of Ran proteins was constructed using MEGA5 software. […]

Pipeline specifications

Software tools PeptideMass, DNAMAN
Databases ExPASy
Applications Phylogenetics, Protein sequence analysis
Organisms Arabidopsis thaliana
Chemicals 2,4-Dichlorophenoxyacetic Acid