Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jul 31 2009
Last update date: Aug 28 2018
Access: Public
Dataset link C2C12 culture: myogenesis timecourse and shRp58 treatment

Experimental Protocol


C2C12 murine skeletal muscle cells were purchased from American Type culture Collection (ATCC). These cells were mainteined in GM (DMEM supplemented with 10% FBS). Cells were grown in GM and after reaching counfluence, the medium was switched to DM (DMEM supplemented with 2% hourse serum) and further incubated. The medium was changed every 2 days. Culture was performed by using within five passages cells. For the experiment of shRNA for Rp58, transfection was performed by using Lipofectamin 2000 (Invitrogen). Stable transfectants were obtained by selection of the transfected C2C12 cells for two weeks. Microarray analysis - RNA was isolated as described from C2C12, and cRNA was synthesized. 10 ug of cRNA were hybridized to Affymetrix mouse 430 2.0 arrays. Intensity values were quantified using RMA algorithm. MAPPFinder (www.genmapp.org) was used to integrate expression data with known pathways.

Repositories


GEO

GSE12993

ArrayExpress

E-GEOD-12993

BioProject

PRJNA112605

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Contact


Hiroshi Asahara