Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 6
Release date: May 28 2018
Last update date: Nov 29 2018
Access: Public
Diseases: Sleep Initiation and Maintenance Disorders
Chemicals: Tyrosine
Dataset link Gene expression profiling study by RNA-seq for identifying genes associated with epithelial-mesenchymal transition and acquired resistance to ALK inhibitors

Experimental Protocol


RNA-seq data of 6 samples, two H3122 parental cells, two ceritinib-treated resistant cells, and two non-resistant cells that combinely treated with certinib and panobinostat, were generated. Total RNA was isolated by RNeasy Mini Kit (Qiagen, CA, USA), according to the manufacturer's protocol. The quality and integrity of the RNA were confirmed by agarose gel electrophoresis and ethidium bromide staining, followed by visual examination under ultraviolet light. Sequencing library was prepared using TruSeq RNA Sample Preparation kit v2 (Illumina, CA, USA) according to the manufacturer’s protocols. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmented, and converted into cDNAs. Then, adapters were ligated and the fragments were amplified on a PCR. Sequencing was performed in paired end reads (2x75 bp) using NextSeq 500 platform (Illumina).

Repositories


GEO

GSE81484

ENA

SRP075253

BioProject

PRJNA321747

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Contact


Seon-Kyu Kim