Computational protocol: Visualization of a DNA-PK/PARP1 complex

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Protocol publication

[…] The DNA-PKcs/Ku70/Ku80/PARP1 sample was applied to carbon-coated grids, negatively stained with 1% uranyl acetate and observed in a JEOL 1200EX electron microscope operating at 100 kV. Micrographs were recorded at a calibrated magnification of 34 000× under low-dose conditions and digitized with a Nikon Coolscan scanner. A total of 21 000 molecular images were extracted with the Boxer program from the EMAN package, processed and refined with a combination of EMAN (), IMAGIC-5 () and Spider () routines. 3D fitting experiments were performed using Chimera (). […]

Pipeline specifications

Software tools EMAN, IMAGIC, SPIDER
Application cryo-EM
Chemicals Poly Adenosine Diphosphate Ribose