|Dataset type:||Genome variation profiling by SNP array, SNP genotyping by SNP array, comparative genomic hybridization by array, genotyping by array, Variation|
|Number of samples:||8|
|Release date:||Dec 16 2010|
|Last update date:||May 17 2017|
|Diseases:||Neoplasms, Genetic Predisposition to Disease|
|Dataset link||Genetic variegation of clonal architecture and stem cells in leukaemia|
Mononuclear cells were isolated at diagnosis of ALL for patient #3 and #7. Transplantation of 2 000 - 2 000 000 unsorted leukaemia cells was performed by intra-tibial injection into 7 - 14 week old NOD/SCID IL2Rgamma deficient mice. Mice were sacrificed when peripheral blood engraftment was >2%. An equivalent of 2 000 - 200 000 CD45 cells were used for serial transplantation. DNA was extracted from mononuclear cells at initial diagnosis and after second transplantation. Copy number analysis of Affymetrix 500K SNP arrays was performed in CNAG 3.0 for patients #3 and #7 at initial diagnosis and after second transplantation. Reference files were 9 samples from leukemia in remission.
Frederik van Delft