|Application:||Gene expression microarray analysis|
|Number of samples:||22|
|Release date:||Nov 25 2014|
|Last update date:||Nov 26 2014|
|Dataset link||Host cell gene and pathway changes associated with high baculovirus in vitro production yields|
To investigate host insect gene responses to baculovirus infection under different conditions, a set of custom microarray probes, based on de novo assembly of target sequences from deep transcriptome sequencing (SRA048534; GEO accession number: GSE34418), were used to simultaneously investigate expression of 8760 Helicoverpa zea insect genes and 134 H. armigera nucleopolyhedrovirus (HearNPV, Accession: NC_002654)) genes. In this study‚ 22 Agilent arrays (from 3 slides of 8x15,000 SurePrint Agilent format) were used to analyze samples from: 4 infections of a so called good clone generating high OB yields (duplicate cultures at 0 hour before infection (h.b.i) and at 18 h.p.i)) and 4 corresponding infections of a bad clone (poor OB yields); 6 infections of cells grown in different media (Sf900III plus FBS, IPL plus YL, and Grace's plus FBS) and 8 samples of infections conducted at low vs. high cell densities in SF900III medium, (2 replicates for each time-point‚ 0 h.p.i and 18 h.p.i‚ and for cell densities 5x10^5 and 2x10^6 cells/ml).