Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Jun 2 2015
Last update date: Mar 8 2018
Access: Public
Genes: GFI1, POU4F3, ATOH1, MYO7A
Dataset link Analyses of iHC transcriptome profiles

Experimental Protocol


Embryoid bodies (EBs) derived from the iGPA-Myo7a:mVenus reporter ESC line at day 8 and day 12, treated with Dox alone or with Dox and RA, were used to obtain FACS-purified populations of iHCs. Unsorted EBs from the same reporter line, grown in the same conditions but without Dox or RA treatment, were used as controls. Independent RNA preparations from each of the selected time points and treatments (three biological replicates for Dox or Dox+RA treatments, two for “no treatment”) were processed and hybridized on Affymetrix whole-transcript microarrays (Mouse Genome 2.1 ST Arrays Strip).

Repositories


GEO

GSE60352

BioProject

PRJNA258081

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Contact


Aida Costa

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