Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Dec 1 2012
Last update date: Aug 6 2018
Access: Public
Diseases: Hamartoma Syndrome, Multiple, Neoplasms
Chemicals: Glucose, Glutamic Acid
Dataset link Systemic elevation of PTEN induces a tumor suppressive metabolic state

Experimental Protocol


In order to elucidate the pathophysiological impact of PTEN elevation, we generated transgenic mice carrying additional copies of this critical tumor suppressor gene (referred to as Super-PTEN mice). In order to maintain the regulation properties of the endogenous Pten gene, we made use of large genomic fragments containing the entire Pten locus carried by BACs (Bacterial Artificial Chromosomes). We next generated mouse embryonic fibroblasts (MEFs) and confirmed successful overexpression of PTEN by the BAC transgenic system. Primary cells derived from Super-PTEN mice represent a powerful tool to elucidate the molecular mechanisms underlying dose-dependent PTEN actions. We therefore performed microarray analysis in primary cells (MEFs) derived from day 13.5 embryos obtained by crossing Super-PTEN mice with C57BL6 mice. Three independent embryos from each genotype were analyzed (background: >98%C57BL6 / CBA). Gene expression profile analysis in these cells will reveal target genes and pathways differentially regulated upon PTEN elevation.

Repositories


GEO

GSE35670

ArrayExpress

E-GEOD-35670

BioProject

PRJNA152443

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Contact


BIDMC Genomics