Computational protocol: AQP5-1364A/C polymorphism and the AQP5 expression influence sepsis survival and immune cell migration: a prospective laboratory and patient study

Similar protocols

Protocol publication

[…] For flow cytometry (FACS) cell suspensions were collected by a lavage of the peritoneum with 5 ml PBS containing 5 mM EDTA. For FACS analysis, cells were stained with the appropriate antibodies (APC antimouse Ly6g/Ly6C (Gr-1) clone RB6-8C5, BioLegend, San Diego, CA, USA; Antimouse CD11c FITC, clone N418, eBioscience, Frankfurt, Germany), after blocking FcRs with anti-CD16/32. Cells were analyzed on a BD FACSCanto II (Heidelberg, Germany). Data were analyzed with Flowing Software 2. [...] Continuous variables are summarized as boxplots. For the comparison of continuous variables of two independent groups, we used exact Wilcoxon–Mann–Whitney tests; for two dependent groups exact Wilcoxon signed-rank tests. The time-to-event survival variable was summarized as the Kaplan–Meier estimator. The reported p-values are two-sided and within each panel adjusted for multiplicity by Bonferroni’s correction. A p value of <0.05 was regarded as statistically significant. All statistical analyses were done using GraphPad Prism 6 (La Jolla, CA, USA) or IBM SPSS Statistics 21. […]

Pipeline specifications

Software tools Flowing Software, SPSS
Applications Miscellaneous, Flow cytometry