Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 9
Release date: May 3 2018
Last update date: May 5 2018
Access: Public
Taxon: Homo sapiens
Dataset link Human 5′ UTR design and variant effect prediction from a massively parallel translation assay

Experimental Protocol


Polysom profiling and sequencing was performed using a library of 300,000 randomized 5' UTR 50-mers with eGFP used as the CDS. Three RNA chemistries were tested: unmodified, pseudouridine, and 1-methylpseudouridine. These were performed in duplicate (6 samples total). A designed library that includes human 5' UTRs, SNVs, and sequences engineered with a genetic algorithm was used with the eGFP CDS (no duplicate). A second randomized library used mCherry as the CDS, also performed in duplicate.

Repositories


GEO

GSE114002

ENA

SRP144485

BioProject

PRJNA454863

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Contact


Paul Sample