Computational protocol: Effect of IVIG Formulation on IgG Binding to Self- and Exo- Antigens In Vitro and In Vivo

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Protocol publication

[…] The phagocytosis assay was performed with the BV-2 microglial cell line (Dr. V. Bocchini, University of Perugia (Perugia, Italy) []) according to Webster et al. [] with some modifications []. In addition to Privigen Fc fragment, which does not mediate phagocytosis, Cytochalasin D (CytoD), which blocks receptor-mediated endocytosis, was used as control. Propidium iodide staining was performed to determine the number of dead cells. After incubation the cells were subjected to FACS analysis to determine FITC-fluorescence intensity (BD FACSdiva; BD Biosciences, Franklin Lakes, NJ, USA) and analyzed with FlowJo (Tree Star Inc., Ashland, OR, USA). Each Experiment was performed at least three times. […]

Pipeline specifications

Software tools BD FACSDiva, FlowJo
Databases CytoD
Application Flow cytometry
Diseases Alzheimer Disease