Computational protocol: Effective heritable gene knockdown in zebrafish using synthetic microRNAs

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Protocol publication

[…] To select target sequences, we mainly used the BLOCK-IT RNAi Designer website from Invitrogen that is optimized for artificial miRNA-mediated knockdown ( Among other tools and websites, we used also GenScript's siRNA Design Center (, siSearch (, IDT SciTools RNAi Design (, Target Finder supplied by Ambion (, Dharmacom RNAi design centre ( and siSPOTR tools ( Fish ( is also useful to obtain complete and accurate 3′-UTR sequences of a gene of interest in zebrafish. […]

Pipeline specifications