Gene Expression profiling in luminal breast cancer cells treated with a novel KDM5 inhibitor, 5-aza-2' Deoxycytidine, or both.

Recently, the H3K4 demethylase, KDM5B, was shown to be amplified and overexpressed in luminal breast cancer, suggesting it might constitute a potential cancer therapy target. Here, we characterize, in breast cancer cells, the molecular effects of a recently developed small-molecule inhibitor of the KDM5 family of proteins, either alone, or in combination with the DNA demethylating agent 5-aza-2’ deoxycytidine (DAC). To determine the effects of these compounds on global gene expression, we used the Agilend Whole Human Genome 4x44k v2 Microarray. We found that alone, the KDM5 inhibitor (KDM5i) increased expression of a small number of genes, but when combined with DAC, the drug enhanced the effects of the latter for increasing expression of hundreds of DAC responsive genes.

Dataset features

Information

Specifications

Application:	Gene expression microarray analysis
Number of samples:	15
Release date:	Dec 8 2017
Last update date:	Mar 9 2018
Access:	Public
Diseases:	Breast Neoplasms
Dataset link	Gene Expression profiling in luminal breast cancer cells treated with a novel KDM5 inhibitor, 5-aza-2' Deoxycytidine, or both.

Experimental Protocol

Two-condition experiment, Mock v Treated Cells. Biological replicates: 3. Total samples: 15

Repositories

GEO

GSE97482

BioProject

PRJNA381957

Dataset features

Specifications

Experimental Protocol

Repositories

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