Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 59
Release date: Aug 16 2012
Last update date: Aug 17 2012
Access: Public
Diseases: Carcinoma, Hepatocellular
Dataset link LncRNA expression profiles of 59 tumorous liver tissues derived from patients with hepatocellular carcinoma (HCC)

Experimental Protocol


For these experiments, we used RNA extracted from 59 HCC tissues and 20 normal livers. Total RNAs from the 20 normal livers were pooled and used as a reference for all microarray experiments. For each microarray experiment, Cy5-labeled probes derived from the DNase-treated total RNA from each HCC sample was hybridized against Cy3-labeled probes derived from common reference on Arraystar Human LncRNA Microarray (Arraystar, Rockville, USA). LncRNAs whose expression was significantly associated with disease-specific survival and time to recurrence were selected based on microarray data. The univariate Cox proportional hazards model was used to assess the association of lncRNAs with survival. We computed a statistical significance level (P value) for two endpoints—the time to cancer-related death and time to recurrence, based on univariate Cox proportional hazards models in BRB-ArrayTools version 4.2.0.

Repositories


GEO

GSE40144

ArrayExpress

E-GEOD-40144

BioProject

PRJNA172922

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Contact


Wei Li