Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 50
Release date: Jul 17 2012
Last update date: Jun 14 2018
Access: Public
Diseases: Alzheimer Disease, Neurodegenerative Diseases
Dataset link Proteogenomics of synaptosomal mitochondrial oxidative stress

Experimental Protocol


mRNA was extracted from synaptosome samples of individual mice from each genotype/treatment group (N=9-11 per group/treatment). 200ng of the purified total RNA was then amplified one round using Ambion’s Illumina RNA Amplification Kit, to prepare cRNA for labeling and hybridization to Illumina’s MouseRef-8 v2.0 expression bead chips as per the manufacturers instructions (Illumina, San Diego, CA, USA).

Repositories


GEO

GSE39372

ArrayExpress

E-GEOD-39372

BioProject

PRJNA170733

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Contact


Simon Melov