Dataset features

Specifications


Application: ChIP-on-chip analysis
Number of samples: 7
Release date: Dec 2 2011
Last update date: Mar 22 2012
Access: Public
Genes: GRHPR
Taxon: Corynebacterium glutamicum
Dataset link Chip-chip from C. glutamicum wild type expressing GlxR tagged with Strep Tag II and cyaB deletion strain expressing GlxR tagged with Strep Tag II.

Experimental Protocol


To identify the direct GlxR targets, we immunoprecipitated DNA from a strain expressing a Strep-tag II-tagged GlxR-protein using an anti-Strep-tag II antibody. To investigate effect of depletion of cAMP by deletion of the cyaB gene, which encodes the sole adenylate cyclase in C. glutamicum, on GlxR binding in vivo, we immunoprecipitated DNA from a cyaB deletion strain expressing a Strep-tag II-tagged GlxR-protein using an anti-Strep-tag II antibody. Three or more independent biological replicates were generated in both cases.

Repositories


GEO

GSE26870

ArrayExpress

E-GEOD-26870

BioProject

PRJNA136001

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Contact


Masayuki Inui
Hideaki Yukawa