Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 33
Release date: Jun 21 2014
Last update date: Sep 22 2016
Access: Public
Diseases: Parkinson Disease, Tooth Loss
Chemicals: Dopamine, Oxidopamine
Dataset link Time Course of Gene Expression in the Substantia Nigra in Response to Intrastriatal 6-hydroxydopamine in the rat.

Experimental Protocol


Tissue was collected from the substantia nigra of 6OHDA treated, sham and naïve rats at 1, 2, 4, 6 & 16 weeks post-treatment. A unilateral striatal lesion was employed that results in a 99% striatal DA reduction and an 80% loss of DA neurons in the substantia nigra. 6OHDA lesions were confirmed via catecholamine quantitation by HPLC. RNA integrity was assessed with an Agilent 2100 Bioanalyzer with minimum RIN acceptance criteria of 8.0. Multidimensional plots and Pearson Correlations were used to establish group/treatment homogeneity. Tissue was subjected to comprehensive gene expression analysis using the Affymetrix Rat Gene 1.0ST array.

Repositories


GEO

GSE58710

ArrayExpress

E-GEOD-58710

BioProject

PRJNA253211

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Contact


Jack Lipton
Jack W Lipton

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