Dataset features

Specifications


Dataset type: Expression profiling by array, Other, Genome binding/occupancy profiling by array
Number of samples: 26
Release date: Sep 21 2010
Last update date: Feb 21 2017
Access: Public
Chemicals: Galactose
Dataset link A Med15 - Hrp1 complex associates with fission yeast Mediator

Experimental Protocol


Genome-wide binding of the Mediator subunit Med15 was analysed using a ChIP-on-chip approach. a-myc ChIP DNA purified from a Med15-myc strain was amplified, labeled and hybridised onto Eurogentec IGR+ORF arrays together with input DNA from the same sample. Similarly, global H3 density was analysed using an anti-H3 antibody for ChIP-on-chip. The analysis of histone density was carried out in three strains: wildtype (711) and knockout strains lacking either Med15 or Hrp1. Furthermore, expression analysis of wildtype, delta-Med15 and delta-Hrp1 strains was carried out using Affymetrix arrays. In each case, 2-3 biological replicates were analysed, and for the Eurogentec experiments dye-swap was always performed.

Repositories


GEO

GSE10079

BioProject

PRJNA108643

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Contact


Claes Gustafsson