Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 66
Release date: Jan 7 2010
Last update date: Feb 15 2013
Access: Public
Dataset link Genome-wide analysis of SATB1 target genes in human T helper cells

Experimental Protocol


Set 1: Total RNA obtained from human CD4+ T cells isolated from peripheral blood transfected with SATB1-shRNA or scramble control plasmids and cultured in Th1 and Th2 conditions for 0, 24 and 48 h (Thp - non antigen exposed precursor). Set 2: Total RNA obtained from human CD4+ T cells isolated from peripheral blood transfected with SATB1-shRNA or scramble control plasmids and cultured in Th1 and Th2 conditions for 0, 24 and 48 h (Thp - non antigen exposed precursor). Set 3: Total RNA obtained from human CD4+ T cells isolated from umbilical cord blood transfected with SATB1-siRNA or scramble control siRNAs and cultured in Th0, Th1 and Th2 conditions for 0, 12, 24 and 48 h (Thp - non antigen exposed precursor). Set 4: Total RNA obtained from human CD4+ T cells isolated from umbilical cord blood transfected with SATB1-siRNA or scramble control siRNAs and cultured in Th0, Th1 and Th2 conditions for 0, 12, 24 and 48 h (Thp - non antigen exposed precursor). Set 5: Total RNA obtained from human CD4+ T cells isolated from umbilical cord blood transfected with SATB1-siRNA or scramble control siRNAs and cultured in Th0, Th1 and Th2 conditions for 24 h.

Repositories


GEO

GSE17241

BioProject

PRJNA118963

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Contact


Helena Ahlfors