|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Jul 31 2015|
|Last update date:||Nov 3 2015|
|Taxon:||Homo sapiens, Anguilla anguilla|
|Dataset link||Transcriptomic profiling of male European eel (Anguilla anguilla) livers at sexual maturity|
A comparative analysis of gene expression was conducted between sexually mature and immature males. Sample labelling and hybridization were conducted following the details in Pujolar et al. (2012). Hybridized slides were scanned at 5 µm resolution using an Agilent DNA microarray scanner. Slides were scanned at two different sensitivity levels (XDR Hi 100% and XDR Lo 10%) and the two linked images generated were analysed together. Data were extracted and background subtracted using the standard procedure in Agilent Feature Extraction (FE) software v. 9.5.1. Hybridization success was evaluated using flag values, excluding those intensities not equal to 1.