Dataset features

Specifications


Application: MNase-seq analysis
Number of samples: 21
Release date: Nov 1 2018
Last update date: Nov 3 2018
Access: Public
Genes: SUPT6H, GTF2B
Dataset link Spt6 is required for the fidelity of promoter selectivity

Experimental Protocol


Transcription start site sequencing for two replicates each of wild-type and spt6-1004 S. cerevisiae strains after an 80 minute shift from 30ºC to 37ºC. ChIP-nexus of TFIIB in two replicates each of wild-type and spt6-1004 strains after an 80 minute shift from 30ºC to 37ºC. Native elongating transcript sequencing of a wild-type strain after an 80 minute shift from 30ºC to 37ºC (two replicates), and spt6-1004 strains grown at 30ºC or shifted to 37ºC for 80 minutes (two replicates each). Two replicates of ChIP-nexus of Spt6 in a wild-type strain grown at 30ºC. Two replicates of ChIP-nexus of Rpb1 in a wild-type strain grown at 30ºC. MNase-sequencing of wild-type (one experiment) and spt6-1004 strains (two replicates) shifted from 30ºC to 37ºC for 80 minutes.

Repositories


GEO

GSE115775

ENA

SRP150458

BioProject

PRJNA475988

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Contact


Fred Winston