|Application:||Gene expression microarray analysis|
|Number of samples:||30|
|Release date:||Aug 27 2009|
|Last update date:||Oct 29 2018|
|Dataset link||Antiestrogen-resistant subclones of MCF-7 human breast cancer cells are derived from a common clonal drug-resistant progenitor|
We picked well-isolated colonies from polyclonal MCF-7 cell culture to establish sublines. All sublines characterized were confirmed as monoclonal by their homogeneous morphology. The unselected anti-estrogen-sensitive U-series sublines were derived from colonies that emerged in low-density MCF-7 cultures without drug pressure. The anti-estrogen-resistant sublines were isolated by exposing high-density MCF-7 cultures to fulvestrant (Fv, 10–100 nM) or 4-hydroxytamoxifen (Tam, 1M) for 21 days. Approximately 1 in 10^5 to 2 X 10^6 anti-estrogen-exposed cells survived this drug pressure. After a 7-day drug-free recovery period, colonies were picked to establish the Tam-selected T-series and the Fv-selected F-series sublines. All T/F-sublines were maintained in the absence of anti-estrogens and were characterized within 20 population doublings.