Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Aug 27 2009
Last update date: Oct 29 2018
Access: Public
Diseases: Breast Neoplasms
Chemicals: Estrogens, Tamoxifen
Dataset link Antiestrogen-resistant subclones of MCF-7 human breast cancer cells are derived from a common clonal drug-resistant progenitor

Experimental Protocol


We picked well-isolated colonies from polyclonal MCF-7 cell culture to establish sublines. All sublines characterized were confirmed as monoclonal by their homogeneous morphology. The unselected anti-estrogen-sensitive U-series sublines were derived from colonies that emerged in low-density MCF-7 cultures without drug pressure. The anti-estrogen-resistant sublines were isolated by exposing high-density MCF-7 cultures to fulvestrant (Fv, 10–100 nM) or 4-hydroxytamoxifen (Tam, 1M) for 21 days. Approximately 1 in 10^5 to 2 X 10^6 anti-estrogen-exposed cells survived this drug pressure. After a 7-day drug-free recovery period, colonies were picked to establish the Tam-selected T-series and the Fv-selected F-series sublines. All T/F-sublines were maintained in the absence of anti-estrogens and were characterized within 20 population doublings.

Repositories


GEO

GSE14986

ArrayExpress

E-GEOD-14986

BioProject

PRJNA111897

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Contact


Ben Wittner