Homotypic cell competition regulates proliferation and tiling of zebrafish pigment cells during colour pattern formation
The adult striped pattern of zebrafish is composed of melanophores, iridophores and xanthophores arranged in superimposed layers in the skin. Previous studies have revealed that the assembly of pigment cells into stripes involves heterotypic interactions between all three chromatophore types. Here we investigate the role of homotypic interactions between cells of the same chromatophore type. Introduction of labelled progenitors into mutants lacking the corresponding cell type allowed us to define the impact of competitive interactions via long-term in vivo imaging. In the absence of endogenous cells, transplanted iridophores and xanthophores show an increased rate of proliferation and spread as a coherent net into vacant space. By contrast, melanophores have a limited capacity to spread in the skin even in the absence of competing endogenous cells. Our study reveals a key role for homotypic competitive interactions in determining number, direction of migration and individual spacing of cells within chromatophore populations.
[…] Repeated imaging of zebrafish was performed as described. Images were acquired on Zeiss LSM 780 NLO confocal and Leica M205 FA stereo-microscopes. ImageJ, Adobe Photoshop, Adobe Illustrator and Imaris were used for image processing and analysis. Maximum intensity projections of confocal scans of the fluorescent samples were uniformly adjusted for brightness and contrast. Scans of the bright field were stacked using “stack focuser” plugin and tile scans were stitched in Fiji. […]
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