Computational protocol: Homotypic cell competition regulates proliferation and tiling of zebrafish pigment cells during colour pattern formation

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Protocol publication

[…] Repeated imaging of zebrafish was performed as described. Images were acquired on Zeiss LSM 780 NLO confocal and Leica M205 FA stereo-microscopes. ImageJ, Adobe Photoshop, Adobe Illustrator and Imaris were used for image processing and analysis. Maximum intensity projections of confocal scans of the fluorescent samples were uniformly adjusted for brightness and contrast. Scans of the bright field were stacked using “stack focuser” plugin and tile scans were stitched in Fiji. […]

Pipeline specifications

Software tools ImageJ, Adobe Illustrator, Imaris
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Danio rerio