Computational protocol: A new cryptic species of Nagiella Munroe from China revealed by DNA barcodes and morphological evidence (Lepidoptera, Crambidae, Spilomelinae)

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Protocol publication

[…] Sequence alignment was carried out by using MUSCLE algorithm implemented in MEGA 6.0 (). MEGA 6.0 was also used to perform genetic distances under the Kimura 2-parameter model of base substitution, to produce the Neighbor-Joining (NJ) tree, and to perform bootstrap analysis (1000 replicates) (). In the present study, we included four sequences of Nagiella inferior and selected Patania ruralis (Scopoli, 1763) as the primary out-group to build the tree which is most closely related genus. Sequences obtained from the current study were deposited in GenBank, in addition to being available in the BOLD dataset DS-PLEQUA. […]

Pipeline specifications

Software tools MUSCLE, MEGA
Application Nucleotide sequence alignment