Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jun 8 2018
Last update date: Oct 29 2018
Access: Public
Diseases: Brain Diseases, Metabolic, Cardiovascular Diseases, Colonic Neoplasms, Neoplasms
Chemicals: Polyphenols
Dataset link Gene expression after 24h Cocoa Extract incubation in HT29 cells

Experimental Protocol


HT29 cells (300,000) were plated and the following day they were treated in triplicate with either saline (control) or 500 µg PCE for 24 hours. After the incubation Total RNA was extracted using Qiagen RNAeasy minikit. Gene expression was analyzed by hybridization to the GeneChip® Human Genome U133 PLUS 2.0 microarrays from Affymettrix containing over 54,700 transcripts and variants. Microarray results were analyzed using the GeneSpring GX software.

Repositories


GEO

GSE94154

BioProject

PRJNA369095

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Contact


Carlos Ciudad