Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jun 24 2011
Last update date: Jun 14 2018
Access: Public
Genes: KLF4, LuxS, CDKN1B
Dataset link Krüppel-like factor 4 regulates T cell function

Experimental Protocol


Klf4fl/fl mice (Katz et al., 2002) were crossed with CD4-Cre mice (Lee et al., 2001) to generate Klf4fl/fl-CD4-Cre+ (Klf4 conditional knockout in T cells) and Klf4fl/fl-CD4-Cre- (control) mice. Mice used for experiments were between 8 and 10 weeks old. Total RNA was extracted from freshly isolated DP+SP4 thymocytes from 3 KO mice and 3 control mice. RNA samples were labeled and hybridized to Illumina Sentrix MouseRef-8 v2 bead arrays.

Repositories


GEO

GSE24880

ArrayExpress

E-GEOD-24880

BioProject

PRJNA132021

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Contact


Kevin Becker
Kevin G Becker