Publication for FancyGene
[…] nested race pcr with pcr anchor primer or generacertm 5′ nested primer. for real-time pcr, 8843-1 primer pair is specific against 3’ region of agaqp1a, and 8843-2 primer pair is against 3’ agaqp1b., fancygene  was used to present gene structures on the genome. alignments of amino-acid sequences were performed using clustalw . prediction of transmembrane domains was performed using […]
It does not read GFF file format properly. I had to manually alter the features of the GFF file to make it work. Ex. I had to change "five_prime_UTR" to "utr", "CDS" to "exon", "three_prime_UTR" to "utr".
You cannot use any genomic positions greater than 5,000,000 or you get an error
Error: Can't use string ("5000000") as an ARRAY ref while "strict refs" in use at FancyGene/Errors.pm line 218, <fh00001Os_WRKY_genes2.gff3.txt> line 38.
Bug reporting does not work. I tried reporting the bug and I got the following error:
The requested URL /~drambald/cgi-bin/fancygene/bugreport.pl was not found on this server.
Apache/2.2.22 (FreeBSD) PHP/5.4.3 proxy_html/3.1.2 mod_ssl/2.2.22 OpenSSL/0.9.8q DAV/2 Server at bio.ieo.eu Port 80